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    The antidepressant effects of asperosaponin Ⅵ are mediated by the suppression2020-04-02

    The antidepressant effects of asperosaponin Ⅵ are mediated by the suppression
    of microglial activation and reduction of TLR4/NF-?B induced IDO expression


    2.2 LPS administration and pharmacological intervention


    The asperosaponin Ⅵ (ASA Ⅵ) standard (purity = 99.92%) was 
    purchased from Chengdu Alfa Biotechnology Co., Ltd.
    The 
    asperosaponin Ⅵ was dissolved in saline. To produce the model, 
    lipopolysaccharide (LPS) (0.83 mg/kg) was administered 
    intraperitoneally to mice. The control mice were administered with 
    author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
    equivoluminal saline. Both control mice and model mice were received 
    ASA Ⅵ (10 mg/kg, 20mg/kg, 40mg/kg and 80mg/kg, i.p.) thirty minutes 
    prior to LPS injection. Following the behavioral analysis,
    immunocytochemistry, RT-PCR analysis, ELISA and western blot 
    analysis were performed.

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