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    A comparative study between Chinese propolis and Brazilian green propolis2020-06-08

    A comparative study between Chinese propolisand Brazilian green propolis: metabolite profile
    and bioactivity


    Among different types, Chinese propolis (ChPs) and Brazilian green propolis (BrGPs) have been shown to
    contain multi-functional properties. Despite extensive research in the field, reports comparing propolis
    from different geographical areas are still limited, compromising our current understanding of the potential
    therapeutic effect associated with propolis and its derived compounds. Herein, a comparative study
    between ChPs and BrGPs including their metabolite profile and bioactivities was performed. Interestingly,
    even when ChPs and BrGPs showed similar anti-inflammatory potential, our results showed that they
    contained very different levels of ethanol extract, total flavonoids and total phenolic acids and in fact,
    LC-MS metabolic profiling and pattern recognition could effectively distinguish ChPs and BrGPs.
    Moreover, all the propolis samples tested showed good anti-oxidant activity and no significant difference
    of free radical scavenging capacity existed between ChPs and BrGPs. In conclusion, ChPs and BrGPs have
    a distinct chemome, but their antioxidant and anti-inflammatory activities are similar.

    2 Materials and methods
    2.1 Reagents and chemicals

    Methanol (HPLC grade) was supplied by Merck Chemicals
    (Darmstadt, Germany). Formic acid and Folin–Ciocalteu
    phenol reagent were from Sigma-Aldrich (St Louis, MO, USA).
    Ethanol (95%, analytical grade) was purchased from
    Guangzhou Chemical Reagents Factory (Guangzhou, China).
    Reference substances of chlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid C,
    caffeic acid phenethyl ester, myricetin, quercetin, kaempferol, apigenin, pinocembrin and galangin
    were supplied by Chengdu Alfa Biotechnology Co., Ltd (Chengdu, Sichuan, China
    ). Artepillin
    C was isolated in our lab from the ethanol soluble fraction of
    Brazilian green propolis, and the purity of which was determined as 98.2% by analytical HPLC, as well as its chemical
    structure was confirmed by HRMS, 1H NMR and 13C NMR (see Fig. S1 in the ESI?).

    Copyright ? 2017-2020 Alle Rechte vorbehalten.

    Technische Unterstützung:库价化学(KuuJia)
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